REVITILISATION OF NON VITAL TOOTH USING PLATELET RICH PLASMA ( PRP )

Initially the tooth to be treated should be administrated with local anesthesia access cavity is prepared. On entry into the root canal. The necrotic pulp should be removed with the aid of a large barbed broach. The working length was determined using an apex locator EX : Elements,sybron endo.) . The canal should be irrigated with approximately 10 ml of 5.25% NaOC1 and dried with paper points. Equal proportions of ciprofloxacin, metronidazole, and minocycine should be ground and mixed with distilled water to a thick paste consistency. This antibiotic mixture should be than placed in the canal using an amalgam carrier and packed with large endodontic pluggers. After which the access cavity was sealed with Cavit ( 3M,ESPE,  France).

A 20-ml sample of whole blood should be drawn from the patient’s right arm for platelet rich plasma (PRP) preparation. PRP is prepared by mixing SmartPReP2 APC+ Autologous Platelet Concentrate+ Procedure Pack (Harvest Technologies Corp, Plymouth, MA) with the Harvest SmartPREP 2 Platelet Concentrate System (Harvest Technologies Corp).

During the next appointment, the teeth should be isolated with a rubber dam, the temporary restoration should be removed, and the antibiotic mixture was washed out by using sterile saline irrigation. The canal was dried with paper points. Prepared PRP should be then injected into the canal space up to the level of the cemento -enamel junction (CEJ) and allowed to clot for 5 minutes. 3mm of white MTA (Pro- Root MTA; Dentsply )was placed directly over the PRP clot. A moist cotton pellet was placed over the MTA and finally restored with GIC ( GC corp,japan.).

                                                REF:JOE,37,2011.