E. faecalis passively maintains pH homeostasis. This occurs as a result of ions penetrating the cell membrane as well as the cytoplasm’s buffering capacity.
E. faecalis has a proton pump that provides an additional means of maintaining pH homeostasis. This is accomplished by “pumping” protons into the cell to lower the internal pH.
At a pH of 11.5 or greater, E. faecalis is unable to survive . However, as a result of the buffering capacity of dentin, it is very unlikely that a pH of 11.5 can be maintained in the dentinal tubules with current calcium hydroxide utilization techniques.
REF: JOE — Volume 32, 2, 2006
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